The RTA Total RNA Kit  provides a straightforward and rapid method for the isolation of up to 40 µg of total RNA from cultured eukaryotic cells, animal (insect, mollusk, bird, fish, reptile, etc.) and human soft tissues. Multiple samples of up to 1×107 eukaryotic cells or 20 mg of tissue can be processed in parallel in fewer than 30 minutes. Purified RNA can be used in many downstream applications such as RT-PCR, Northern blotting, nuclease protection assay, and in vitro translation.

Starting Material10-50 mg Tissue, 200 ul Blood, 1 x 107 Cell
Sample TypeTissue, Blood, Cell (Bacteria, Yeast, Mold and any culture cell)
Protocol Time30 minutes (from tissue)
TechnologySilica Spin Column
Downstream Applications *PCR
*RT-PCR *Cloning
*Next Generation Sequencing
PurityA260:A280 ratio: 1,70-1,90
Yield40 µg RNA from 10-50 mg Tissue samples
ProductReaction size/kit Cat#GTIN
RTA Total RNA Isolation Kit50 test090400508698918035850
RTA Total RNA Isolation Kit100 test090401008698918035867
RTA Total RNA Isolation Kit250 test090402508698918035874